St John's College Oxford
Dr Dominic Alonzi

Dr Dominic Alonzi

Stipendiary Lecturer in Biochemistry

Subjects

Biography

I read Molecular and Cellular Biochemistry at Corpus Christi, Oxford before reading for a DPhil at Linacre College with Dr Terry Butters and Prof Raymond Dwek, CBE, FRS at the Oxford Glycobiology Institute, Department of Biochemistry.

I hope by understanding the structures and function of biomolecules – the energetics, interactions and the regulation of biochemical pathways – we can gain an understanding that can be used to develop therapeutics for a wide range of disease.  

I enjoy supervising students in research projects and lecturing undergraduates in biological chemistry and glycobiology. I give tutorials in a number of subjects: Biochemistry, Biological Sciences, Biomedical Science and Medicine.

Research Interests

My research involves working in the field of glycobiology. Glycobiology has made major contributions to understanding concepts in protein folding, immunology and virology, laying the foundations for applying glycobiology to the development of novel strategies for therapeutics. Inhibitors of the glycosylation pathway can perturb glycan dependent protein folding in the ER with clinically relevant broad-spectrum antiviral properties, with a class of small molecule inhibitors known as iminosugars leading the research in this field. I examine how these small molecule inhibitors can have an effect on a number of cellular targets to enhance and elucidate their therapeutic potential and multiple mechanisms of action.

Publications

Interdomain conformational flexibility underpins the activity of UGGT, the eukaryotic glycoprotein secretion checkpoint.

P. Roversi, L. Marti, A.T. Caputo, D.S. Alonzi, J.C. Hill, K.C. Dent, A. Kumar, M.D. Levasseur, A. Lia, T. Waksman, S. Basu, Y. Soto Albrecht, K. Qian, J.P. McIvor, Lipp, D. Siliqi, S. Vasiljević, S. Mohammed, P. Lukacik, M.A. Walsh, A. Santino, N. Zitzmann. Proc Natl Acad Sci USA. 2017 Aug 8;114(32):8544-8549.

Iminosugar antivirals: the therapeutic sweet spot.

D.S. Alonzi, K.A. Scott, R.A. Dwek, N. Zitzmann. Biochem Soc Trans. 2017 Apr 15;45(2):571-582.

Structures of mammalian ER α-glucosidase II capture the binding modes of broad-spectrum iminosugar antivirals.

A.T. Caputo, D.S. Alonzi, L. Marti, I.B. Reca, J.L. Kiappes, W.B. Struwe, A. Cross, S. Basu, E.D. Lowe, B. Darlot, A. Santino, P. Roversi, N. Zitzmann. Proc Natl Acad Sci USA. 2016 Aug 9;113(32).

Glycoprotein misfolding in the endoplasmic reticulum: identification of released oligosaccharides reveals a second ER-associated degradation pathway for Golgi-retrieved proteins.                                                                 

D.S. Alonzi, N.V. Kukushkin, S.A. Allman, Z. Hakki, S.J. Williams, L. Pierce, R.A. Dwek and T.D. Butters. Cell Mol Life Sci. 2013 Aug;70(15):2799-814.

Glucosylated free oligosaccharides are biomarkers of endoplasmic reticulum alpha-glucosidase inhibition.

D.S. Alonzi, D.C. Neville, R.H. Lachmann, R.A. Dwek and T.D. Butters (2008) Biochem. J., 409, 571-580.